2 edition of Optimizing molecular methods to detect human caliciviruses in environmental samples found in the catalog.
Optimizing molecular methods to detect human caliciviruses in environmental samples
|Statement||prepared by Jan Vinjé ; sponsored by AWWA Research Foundation and U.S. Environmental Protection Agency.|
|Contributions||Vinjé, Jan., AWWA Research Foundation., United States. Environmental Protection Agency.|
|LC Classifications||TD427.V55 .O68 2007|
|The Physical Object|
|Pagination||xviii, 62 p. :|
|Number of Pages||62|
|ISBN 10||1843396211, 1583215794|
|ISBN 10||9781843396215, 9781583215791|
|LC Control Number||2009419121|
A recent comparison study concluded that, at the optimal cut-off points, Hybrid Capture 2 and 3 had similar screening performance characteristics for high-grade lesions diagnosed at the enrolment visit [ 48 ]. The complete nucleotide sequence of the RNA genome of a porcine enteric calicivirus genetically related to Sapporo like viruses. Saif, advisor Figure 1 Figure 1. Thus, the HPV infection is necessary for the development of the cervical cancer.
Direct Detection of Viral Genome Viral genome can be detected directly from specimens without having been grown first in cell culture. Davis, CA, July In this book, a panel of experts distil the most important up-to-date research findings in their respective calicivirus field of study producing timely and comprehensive reviews. Bile acids were identified as the active factor in IC that induced the increased cAMP and down-regulated interferon-mediated STAT 1 activation, a key element in innate immunity to viruses. Int J Hyg Environ Health.
Multiplex HPV genotyping methods based on hybridization to fluorescently labeled beads have also been reported [ 53 ]. Oncogenic HPV types 16 and 18 and history of other concurrent sexually transmitted diseases were found to be significantly associated with progression to cervical cancer [ 5 ]. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice Mason, H. Saif, and K.
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The last one is the second most common cancer among women worldwide [ 1 - 3 ]. The induction of hyperproliferation by the E7 oncoprotein triggers apoptosis, which is blocked by the actions of the E6 oncoprotein.
The eye drops help with the conjunctivitis and can help prevent secondary infections. Vet Microbiol. The most popular amplification method is PCR The technique is based on the use of synthetic oligonucleotides flanking the target nucleic sequence of interest.
In the pup, the norovirus strain was detected in conjunction with a CPV-2a strain that was likely responsible for the severity of the observed clinical signs, since CPV-2 is a major enteric pathogen of dogs. Cloning and sequencing of a porcine enteric calicivirus genetically related to Sapporo-like viruses.
Saif and K.
Besides outbreak situations, molecular epidemiological techniques may be used to study the transmission of a virus worldwide, such as the transmission of the various HIV-1 subtypes throughout the world. Some types of HPV have been established as the central cause of cervical carcinoma [ 4 - 7 ] Acquisition of HPV is very common, particularly among sexually active young adults, and incidence of infection with oncogenic HPV types appears to be higher than the incidence of infection with non-oncogenic types [ 8 ].
The association between HPV and human cancer was first proposed more than three decades ago by Harald zur Hausen, and he was honored with one of two Nobel Prizes in Medicine for his isolation and characterization of HPV 16 in and later HPV 18 in cervical cancer [ 20 ].
At present, we are treating pig RBCs with different enzymes to identify whether the receptors on pig RBCs are proteins or carbohydrates. Although a straightforward culturing method remains lacking for noroviruses, important progress has been made in immunological studies using virus-like particles.
More than HPV types have been identified and about 40 types can infect the genital tract. The remaining viral types are rarely found in malignancies. J Virol. Hemagglutination by Cowden porcine enteric calicivirus and development of a hemagglutination-inhibition test.
Our data revealed that PEC virus replication is dependent on a cAMP signaling pathway induced by IC, a novel mechanism whereby virus growth is dependent on a cell signal transduction pathway induced by the surrounding intestinal microenvironment IC.
Atlanta, Georgia, March, Abst. The best-characterized HPV 16 E6 activity is its ability to induce degradation of the tumor suppressor protein p53 via the ubiquitin pathway.
Due to similarities in genome organization between the different genera, recombination predomoninantly occurs at the start of the major structural gene which encodes the capsid, VP1. A reverse genetics system for porcine enteric calicivirus, a prototype sapovirus in the Caliciviridae.
Publications Guo, M, K.Journal of Virological Methods () – An efﬁcient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples Leera Kittigula,∗, Som Ekchaloemkieta, Fuangfa Utrarachkija, Kanokrat Siripanichgona, Dusit Sujiraratb, Supornvit Pungchittona, Augsana Boonthumc.
Article - "Test and validation of methods to sample and detect human virus from environmental surfaces using norovirus as a model virus" (limited access) Jan 06 Summary: Viruses cause a major proportion of human infections, especially gastroenteritis and respiratory infections in children and adults.
Optimizing Molecular Methods to Detect Human Caliciviruses in Environmental Samples Other compilation by Jan Vinje published on August, hildebrandsguld.com: Jan Vinje: LibrosFormat: Tapa blanda. You have just watched JoVE's video on the detection of bacteriophages in environmental samples.
You should now understand the basic biology of phages, how to perform a plaque assay to quantitate phages in an environmental sample, and how plaque assays can be used to study phages and other viruses in environmental or clinical samples.
Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis.
Optimizing Molecular Methods to Detect Human Caliciviruses in Environmental Samples, Jan Vinje X History of the Knights of Pythias (), Hugh Goold Webb In Search of American Jewish Culture, Stephen J Whitfield Regeneration (), Edmund H. Sears.